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Creators/Authors contains: "Spolek, RL"

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  1. ; ; ; (, FEBS letters)
    Human BRCA2 protects the DNA when replication forks stall, whereas MRE11-RAD50 and WRN-DNA2 process or partially degrade these substrates. When mutated, these genes result in distinct genetic instabilities and cancers, arguing they have unique, not redundant, functions. Escherichia coli encodes functional homologs of MRE11-RAD50 (SbcC-SbcD), WRN-DNA2 (RecQ-RecJ), and BRCA2 (RecF). Here, we use 2-dimensional gels, pulse-labelling, and replication-profiling analysis to show the bacterial homologs act at distinct substrates and loci on the chromosome. Whereas RecF and RecJ-RecQ protect and process DNA at arrested replication forks to facilitate repair, RecBCD and SbcC-SbcD protect and process DNA at sites where forks converge. Comparing the assays used in E. coli to human cells, we consider whether these cellular roles may be functionally conserved. 
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    Free, publicly-accessible full text available October 20, 2026